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Fungal testing

Tuesday, 18 January 2011 12:30 Vinc Neil
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                       Email:   network1@hunterlink.net.au
                                    Fungal Testing Overview
Fungal exposure can lead to health concerns a lot of clinicians are not aware of and as such do not look for any indoor or occupied environmental exposural issues where there are symptomatologies which are reflective of bacterial and fungal exposure.
Current inadequate testing protocols do not show the full extent of the fungal contamination, previously culturing has been carried out on one medium which due to overgrowth along with slow growing fungal species can portray a totally incorrect fungal overview.
Our company along with our consultant Mycologist David Lark now do all testing where required on a 4 plate system which with different agars can restrict overgrowth and assist with slow growing fungal species.
Allowing a far more defined analysis of the contamination.
Fungi are an important part of the indoor contamination (1, 2) but there are other components which also must be tested for, where there are health issues.
Documented research shows that a vast majority of health issues are due to the exposure to the fungal toxins which are called Mycotoxins (3, 4) and an area which has not been dealt with in buildings affected by fungal contamination is the ultrafine particles, consisting of fungal fragments which are not necessarily reduced successfully by cleaning and in those sensitized individuals can cause ongoing health problems (4).
The testing scope becomes extremely important as it has to deal successfully with being able to remediate what is the contamination that the individual has become sensitive to- i.e. if the individual has a sensitivity to toxins or the enzymes then the scope has to be able to deal with addressing that, if there are hidden areas of exposures being in wall cavity, in ceilings , behind or in cupboards and when these are not addressed then the developed health issues will continue to be of concern, even though visible mould may appear to have been removed.
Insurance companies and builders have yet to understand that there is sometimes more to a fungal remediation than just vacuuming and wiping surfaces for visible mould and by utilizing the correct sampling protocols they can be made aware of what is required.
To this end our where there are health concerns we would suggest that the following forms of testing should be carried out:
  1. Fungal culturing with a four plate agar system on all surfaces and within wall cavities, ceilings and soft furnishings to include carpets and under floating floors.
  1. Air cultures with a four plate agar system to include an outdoor reference sample.
  1. Testing of both airborne and surface sampling as per point 1 for fungal Mycotoxins. Currently air sampling is not available for bacterial toxins but that will become available shortly.
  1. Mycotoxins in the exposed individual’s urine which may assist any treating clinicians.
  1. Where there have been biopsy’s taken testing of tissue for Genetic fungal DNA finger printing along with genetic DNA sampling in collected dust which may assist any treating clinicians.
  1. Results from testing in both 5 and 6 can be screened against what has been cultured to determine if there is a correlation between the occupied space and the individual.
1. Proinflammatory Response of Immature Human
Dendritic Cells is Mediated by Dectin-1 after
Exposure to Aspergillus fumigatus Germ Tubes
Markus Mezger,1 Susanne Kneitz,2 Iwona Wozniok,1 Oliver Kurzai,3 Hermann Einsele,1 and Juergen Loeffler1
1Medizinische Klinik und Poliklinik II, 2Interdisziplina¨res Zentrum fu¨r Klinische Forschung, Labor für Microarray-Anwendungen, 3Institut für Hygiene
und Mikrobiologie, Universität Würzburg, Würzburg, Germany.
2.Clinical Microbiology Reviews, January 2003, p. 144-172, Vol. 16, No. 1
0893-8512/03/$08.00+0     DOI: 10.1128/CMR.16.1.144-172.2003
Indoor Mold, Toxigenic Fungi, and Stachybotrys chartarum: Infectious Disease Perspective
D. M. Kuhn1,2,3 and M. A. Ghannoum2,3*
4.Desquamative Interstital Pneumonitis and Diffuse Alveolar Damage in Textile Workers: Potential Rol of Mycotoxins
M.Diane Lougheed, Jean O.Roos.William R.Waddell and Peter W. Munt
Chest 1995;108;1196-1200 DOI 10.1378/cest. 108.5.1196
5. Aerodynamic characteristics and respiratory deposition of
fungal fragments Seung-Hyun Choa, Sung-Chul Seoa, Detlef Schmechelb,
Sergey A. Grinshpuna, Tiina Reponena,_aCenter for Health-Related Aerosol Studies, Department of Environmental Health, University of Cincinnati,
Cincinnati, OH 45267-0056, USA
bAllergy and Clinical Immunology Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health,
Morgantown, WV 26505-2845, USA.
Vince Neil
Mycotox Pty Ltd
Professional Member - Global Indoor Health Network
Ph: 0418 491 507

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Last Updated on Tuesday, 18 January 2011 01:47